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Background and Aims: More and more, consumers are trying to counterbalance their active, hectic lifestyles by seeking for foods and beverages that contribute to healthier living. In response to consumer demand, there is a need for healthful products for products enriched in plant-derived bioactive, health promoting micronutrients, which activity is confirmed on the basis of scientific studies.
Examples: chemopreventive N-methylpyridinium ions (NMP) in roasted coffee (A in Figure), antioxidant and biotransformation enzyme modulating pronyl-L-lysine in bread crust melanoidins (B in Figure); cytotoxic Maillard reaction products formed from carbohydrates and amino acids; stomach irritating roast substances in roast coffee beverages etc.
- Bioactivity-directed screening of complex foods and fractions obtained thereof
- Structure determination by means of LC-MS/MS and 1D-/2D-NMR spectroscopy
- Chemical synthesis of target compounds
- Clarification of reaction pathways leading to formation/degradation of bioactive compounds by means of 13C-labelling experiments in combination with LC-MS/MS and/or 13C NMR spectroscopic detection

Figure Chemical structures of the biotransformation phase II inductor NMP (A) and of the antioxidant pronyl-L-lysine (B).